individual protein band densitometry Search Results


odyssey infrared imaging systems software 2 1  (LI-COR)
99
LI-COR odyssey infrared imaging systems software 2 1
A. Periosteal cell cultures were left untreated or were treated with 1 or 10 μm of LY294002 for 60 minutes. Total cell lysate (100 μg) was electrophoresed on 8 % SDS-PAGE. The blot was probed using anti-phospho AKT Thr 308 antibody (top), and then stripped and reprobed with anti-AKT (bottom) to verify equal loading of protein. The amounts of phosphorylated to total proteins in individual bands were quantified by using Odyssey Infrared Imaging Systems software 2.1 (LICOR Biosciences), and the ratio of pAKT/AKT is indicated. A representative of two experiments is shown. B. Cells left untreated or treated with 40 μm of cycloheximide for 2, 4, 6 and 8 hours. Cell lysate (50 μg) were electrophoresed on 10 % SDS-PAGE. Blots were probed with anti-Osterix-antibodies (top) and anti-GAPDH antibodies (bottom) to verify equal protein loading. C. Bar graphs represent Osterix/GAPDH of three pooled experiments; * p<0.01 Vs. WT. D. Representative 20× fluorescent images of Osterix-expressing WT and YF periosteal cells counterstained with DAPI in the presence and absence of 10μm LY294002. White arrowheads point to cells in which Osterix is localized in the nucleus. E. Bar graphs represent the percent of periosteal cells with nuclear Osterix expression over total number of cells in the absence and presence of 1 and 10 μM LY294002. p* p<0.01 Vs. WT. A representative of 3 experiments is shown.
Odyssey Infrared Imaging Systems Software 2 1, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ltq esi ms/ms  (Thermo Fisher)
90
Thermo Fisher ltq esi ms/ms
A. Periosteal cell cultures were left untreated or were treated with 1 or 10 μm of LY294002 for 60 minutes. Total cell lysate (100 μg) was electrophoresed on 8 % SDS-PAGE. The blot was probed using anti-phospho AKT Thr 308 antibody (top), and then stripped and reprobed with anti-AKT (bottom) to verify equal loading of protein. The amounts of phosphorylated to total proteins in individual bands were quantified by using Odyssey Infrared Imaging Systems software 2.1 (LICOR Biosciences), and the ratio of pAKT/AKT is indicated. A representative of two experiments is shown. B. Cells left untreated or treated with 40 μm of cycloheximide for 2, 4, 6 and 8 hours. Cell lysate (50 μg) were electrophoresed on 10 % SDS-PAGE. Blots were probed with anti-Osterix-antibodies (top) and anti-GAPDH antibodies (bottom) to verify equal protein loading. C. Bar graphs represent Osterix/GAPDH of three pooled experiments; * p<0.01 Vs. WT. D. Representative 20× fluorescent images of Osterix-expressing WT and YF periosteal cells counterstained with DAPI in the presence and absence of 10μm LY294002. White arrowheads point to cells in which Osterix is localized in the nucleus. E. Bar graphs represent the percent of periosteal cells with nuclear Osterix expression over total number of cells in the absence and presence of 1 and 10 μM LY294002. p* p<0.01 Vs. WT. A representative of 3 experiments is shown.
Ltq Esi Ms/Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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online nanolc-ms/ms ultimate 3000 coupled to ltq-orbitrap velos  (Thermo Fisher)
90
Thermo Fisher online nanolc-ms/ms ultimate 3000 coupled to ltq-orbitrap velos
A. Periosteal cell cultures were left untreated or were treated with 1 or 10 μm of LY294002 for 60 minutes. Total cell lysate (100 μg) was electrophoresed on 8 % SDS-PAGE. The blot was probed using anti-phospho AKT Thr 308 antibody (top), and then stripped and reprobed with anti-AKT (bottom) to verify equal loading of protein. The amounts of phosphorylated to total proteins in individual bands were quantified by using Odyssey Infrared Imaging Systems software 2.1 (LICOR Biosciences), and the ratio of pAKT/AKT is indicated. A representative of two experiments is shown. B. Cells left untreated or treated with 40 μm of cycloheximide for 2, 4, 6 and 8 hours. Cell lysate (50 μg) were electrophoresed on 10 % SDS-PAGE. Blots were probed with anti-Osterix-antibodies (top) and anti-GAPDH antibodies (bottom) to verify equal protein loading. C. Bar graphs represent Osterix/GAPDH of three pooled experiments; * p<0.01 Vs. WT. D. Representative 20× fluorescent images of Osterix-expressing WT and YF periosteal cells counterstained with DAPI in the presence and absence of 10μm LY294002. White arrowheads point to cells in which Osterix is localized in the nucleus. E. Bar graphs represent the percent of periosteal cells with nuclear Osterix expression over total number of cells in the absence and presence of 1 and 10 μM LY294002. p* p<0.01 Vs. WT. A representative of 3 experiments is shown.
Online Nanolc Ms/Ms Ultimate 3000 Coupled To Ltq Orbitrap Velos, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantity one software  (Bio-Rad)
96
Bio-Rad quantity one software
A. Periosteal cell cultures were left untreated or were treated with 1 or 10 μm of LY294002 for 60 minutes. Total cell lysate (100 μg) was electrophoresed on 8 % SDS-PAGE. The blot was probed using anti-phospho AKT Thr 308 antibody (top), and then stripped and reprobed with anti-AKT (bottom) to verify equal loading of protein. The amounts of phosphorylated to total proteins in individual bands were quantified by using Odyssey Infrared Imaging Systems software 2.1 (LICOR Biosciences), and the ratio of pAKT/AKT is indicated. A representative of two experiments is shown. B. Cells left untreated or treated with 40 μm of cycloheximide for 2, 4, 6 and 8 hours. Cell lysate (50 μg) were electrophoresed on 10 % SDS-PAGE. Blots were probed with anti-Osterix-antibodies (top) and anti-GAPDH antibodies (bottom) to verify equal protein loading. C. Bar graphs represent Osterix/GAPDH of three pooled experiments; * p<0.01 Vs. WT. D. Representative 20× fluorescent images of Osterix-expressing WT and YF periosteal cells counterstained with DAPI in the presence and absence of 10μm LY294002. White arrowheads point to cells in which Osterix is localized in the nucleus. E. Bar graphs represent the percent of periosteal cells with nuclear Osterix expression over total number of cells in the absence and presence of 1 and 10 μM LY294002. p* p<0.01 Vs. WT. A representative of 3 experiments is shown.
Quantity One Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorochem hd2  (Fluorochem Ltd)
90
Fluorochem Ltd fluorochem hd2
A. Periosteal cell cultures were left untreated or were treated with 1 or 10 μm of LY294002 for 60 minutes. Total cell lysate (100 μg) was electrophoresed on 8 % SDS-PAGE. The blot was probed using anti-phospho AKT Thr 308 antibody (top), and then stripped and reprobed with anti-AKT (bottom) to verify equal loading of protein. The amounts of phosphorylated to total proteins in individual bands were quantified by using Odyssey Infrared Imaging Systems software 2.1 (LICOR Biosciences), and the ratio of pAKT/AKT is indicated. A representative of two experiments is shown. B. Cells left untreated or treated with 40 μm of cycloheximide for 2, 4, 6 and 8 hours. Cell lysate (50 μg) were electrophoresed on 10 % SDS-PAGE. Blots were probed with anti-Osterix-antibodies (top) and anti-GAPDH antibodies (bottom) to verify equal protein loading. C. Bar graphs represent Osterix/GAPDH of three pooled experiments; * p<0.01 Vs. WT. D. Representative 20× fluorescent images of Osterix-expressing WT and YF periosteal cells counterstained with DAPI in the presence and absence of 10μm LY294002. White arrowheads point to cells in which Osterix is localized in the nucleus. E. Bar graphs represent the percent of periosteal cells with nuclear Osterix expression over total number of cells in the absence and presence of 1 and 10 μM LY294002. p* p<0.01 Vs. WT. A representative of 3 experiments is shown.
Fluorochem Hd2, supplied by Fluorochem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gelexpert software  (NucleoTech Corporation)
90
NucleoTech Corporation gelexpert software
A. Periosteal cell cultures were left untreated or were treated with 1 or 10 μm of LY294002 for 60 minutes. Total cell lysate (100 μg) was electrophoresed on 8 % SDS-PAGE. The blot was probed using anti-phospho AKT Thr 308 antibody (top), and then stripped and reprobed with anti-AKT (bottom) to verify equal loading of protein. The amounts of phosphorylated to total proteins in individual bands were quantified by using Odyssey Infrared Imaging Systems software 2.1 (LICOR Biosciences), and the ratio of pAKT/AKT is indicated. A representative of two experiments is shown. B. Cells left untreated or treated with 40 μm of cycloheximide for 2, 4, 6 and 8 hours. Cell lysate (50 μg) were electrophoresed on 10 % SDS-PAGE. Blots were probed with anti-Osterix-antibodies (top) and anti-GAPDH antibodies (bottom) to verify equal protein loading. C. Bar graphs represent Osterix/GAPDH of three pooled experiments; * p<0.01 Vs. WT. D. Representative 20× fluorescent images of Osterix-expressing WT and YF periosteal cells counterstained with DAPI in the presence and absence of 10μm LY294002. White arrowheads point to cells in which Osterix is localized in the nucleus. E. Bar graphs represent the percent of periosteal cells with nuclear Osterix expression over total number of cells in the absence and presence of 1 and 10 μM LY294002. p* p<0.01 Vs. WT. A representative of 3 experiments is shown.
Gelexpert Software, supplied by NucleoTech Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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620 scanning densitometer  (Bio-Rad)
90
Bio-Rad 620 scanning densitometer
A. Periosteal cell cultures were left untreated or were treated with 1 or 10 μm of LY294002 for 60 minutes. Total cell lysate (100 μg) was electrophoresed on 8 % SDS-PAGE. The blot was probed using anti-phospho AKT Thr 308 antibody (top), and then stripped and reprobed with anti-AKT (bottom) to verify equal loading of protein. The amounts of phosphorylated to total proteins in individual bands were quantified by using Odyssey Infrared Imaging Systems software 2.1 (LICOR Biosciences), and the ratio of pAKT/AKT is indicated. A representative of two experiments is shown. B. Cells left untreated or treated with 40 μm of cycloheximide for 2, 4, 6 and 8 hours. Cell lysate (50 μg) were electrophoresed on 10 % SDS-PAGE. Blots were probed with anti-Osterix-antibodies (top) and anti-GAPDH antibodies (bottom) to verify equal protein loading. C. Bar graphs represent Osterix/GAPDH of three pooled experiments; * p<0.01 Vs. WT. D. Representative 20× fluorescent images of Osterix-expressing WT and YF periosteal cells counterstained with DAPI in the presence and absence of 10μm LY294002. White arrowheads point to cells in which Osterix is localized in the nucleus. E. Bar graphs represent the percent of periosteal cells with nuclear Osterix expression over total number of cells in the absence and presence of 1 and 10 μM LY294002. p* p<0.01 Vs. WT. A representative of 3 experiments is shown.
620 Scanning Densitometer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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individual protein bands  (Bio-Rad)
99
Bio-Rad individual protein bands
A. Periosteal cell cultures were left untreated or were treated with 1 or 10 μm of LY294002 for 60 minutes. Total cell lysate (100 μg) was electrophoresed on 8 % SDS-PAGE. The blot was probed using anti-phospho AKT Thr 308 antibody (top), and then stripped and reprobed with anti-AKT (bottom) to verify equal loading of protein. The amounts of phosphorylated to total proteins in individual bands were quantified by using Odyssey Infrared Imaging Systems software 2.1 (LICOR Biosciences), and the ratio of pAKT/AKT is indicated. A representative of two experiments is shown. B. Cells left untreated or treated with 40 μm of cycloheximide for 2, 4, 6 and 8 hours. Cell lysate (50 μg) were electrophoresed on 10 % SDS-PAGE. Blots were probed with anti-Osterix-antibodies (top) and anti-GAPDH antibodies (bottom) to verify equal protein loading. C. Bar graphs represent Osterix/GAPDH of three pooled experiments; * p<0.01 Vs. WT. D. Representative 20× fluorescent images of Osterix-expressing WT and YF periosteal cells counterstained with DAPI in the presence and absence of 10μm LY294002. White arrowheads point to cells in which Osterix is localized in the nucleus. E. Bar graphs represent the percent of periosteal cells with nuclear Osterix expression over total number of cells in the absence and presence of 1 and 10 μM LY294002. p* p<0.01 Vs. WT. A representative of 3 experiments is shown.
Individual Protein Bands, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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peptide mass fingerprinting (pmf) analysis  (Sangon Biotech)
90
Sangon Biotech peptide mass fingerprinting (pmf) analysis
A. Periosteal cell cultures were left untreated or were treated with 1 or 10 μm of LY294002 for 60 minutes. Total cell lysate (100 μg) was electrophoresed on 8 % SDS-PAGE. The blot was probed using anti-phospho AKT Thr 308 antibody (top), and then stripped and reprobed with anti-AKT (bottom) to verify equal loading of protein. The amounts of phosphorylated to total proteins in individual bands were quantified by using Odyssey Infrared Imaging Systems software 2.1 (LICOR Biosciences), and the ratio of pAKT/AKT is indicated. A representative of two experiments is shown. B. Cells left untreated or treated with 40 μm of cycloheximide for 2, 4, 6 and 8 hours. Cell lysate (50 μg) were electrophoresed on 10 % SDS-PAGE. Blots were probed with anti-Osterix-antibodies (top) and anti-GAPDH antibodies (bottom) to verify equal protein loading. C. Bar graphs represent Osterix/GAPDH of three pooled experiments; * p<0.01 Vs. WT. D. Representative 20× fluorescent images of Osterix-expressing WT and YF periosteal cells counterstained with DAPI in the presence and absence of 10μm LY294002. White arrowheads point to cells in which Osterix is localized in the nucleus. E. Bar graphs represent the percent of periosteal cells with nuclear Osterix expression over total number of cells in the absence and presence of 1 and 10 μM LY294002. p* p<0.01 Vs. WT. A representative of 3 experiments is shown.
Peptide Mass Fingerprinting (Pmf) Analysis, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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identification of individual protein bands  (Proteome Factory AG)
90
Proteome Factory AG identification of individual protein bands
A. Periosteal cell cultures were left untreated or were treated with 1 or 10 μm of LY294002 for 60 minutes. Total cell lysate (100 μg) was electrophoresed on 8 % SDS-PAGE. The blot was probed using anti-phospho AKT Thr 308 antibody (top), and then stripped and reprobed with anti-AKT (bottom) to verify equal loading of protein. The amounts of phosphorylated to total proteins in individual bands were quantified by using Odyssey Infrared Imaging Systems software 2.1 (LICOR Biosciences), and the ratio of pAKT/AKT is indicated. A representative of two experiments is shown. B. Cells left untreated or treated with 40 μm of cycloheximide for 2, 4, 6 and 8 hours. Cell lysate (50 μg) were electrophoresed on 10 % SDS-PAGE. Blots were probed with anti-Osterix-antibodies (top) and anti-GAPDH antibodies (bottom) to verify equal protein loading. C. Bar graphs represent Osterix/GAPDH of three pooled experiments; * p<0.01 Vs. WT. D. Representative 20× fluorescent images of Osterix-expressing WT and YF periosteal cells counterstained with DAPI in the presence and absence of 10μm LY294002. White arrowheads point to cells in which Osterix is localized in the nucleus. E. Bar graphs represent the percent of periosteal cells with nuclear Osterix expression over total number of cells in the absence and presence of 1 and 10 μM LY294002. p* p<0.01 Vs. WT. A representative of 3 experiments is shown.
Identification Of Individual Protein Bands, supplied by Proteome Factory AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant egfp standard  (TaKaRa)
95
TaKaRa recombinant egfp standard
A. Periosteal cell cultures were left untreated or were treated with 1 or 10 μm of LY294002 for 60 minutes. Total cell lysate (100 μg) was electrophoresed on 8 % SDS-PAGE. The blot was probed using anti-phospho AKT Thr 308 antibody (top), and then stripped and reprobed with anti-AKT (bottom) to verify equal loading of protein. The amounts of phosphorylated to total proteins in individual bands were quantified by using Odyssey Infrared Imaging Systems software 2.1 (LICOR Biosciences), and the ratio of pAKT/AKT is indicated. A representative of two experiments is shown. B. Cells left untreated or treated with 40 μm of cycloheximide for 2, 4, 6 and 8 hours. Cell lysate (50 μg) were electrophoresed on 10 % SDS-PAGE. Blots were probed with anti-Osterix-antibodies (top) and anti-GAPDH antibodies (bottom) to verify equal protein loading. C. Bar graphs represent Osterix/GAPDH of three pooled experiments; * p<0.01 Vs. WT. D. Representative 20× fluorescent images of Osterix-expressing WT and YF periosteal cells counterstained with DAPI in the presence and absence of 10μm LY294002. White arrowheads point to cells in which Osterix is localized in the nucleus. E. Bar graphs represent the percent of periosteal cells with nuclear Osterix expression over total number of cells in the absence and presence of 1 and 10 μM LY294002. p* p<0.01 Vs. WT. A representative of 3 experiments is shown.
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imagelab  (Bio-Rad)
90
Bio-Rad imagelab
A. Periosteal cell cultures were left untreated or were treated with 1 or 10 μm of LY294002 for 60 minutes. Total cell lysate (100 μg) was electrophoresed on 8 % SDS-PAGE. The blot was probed using anti-phospho AKT Thr 308 antibody (top), and then stripped and reprobed with anti-AKT (bottom) to verify equal loading of protein. The amounts of phosphorylated to total proteins in individual bands were quantified by using Odyssey Infrared Imaging Systems software 2.1 (LICOR Biosciences), and the ratio of pAKT/AKT is indicated. A representative of two experiments is shown. B. Cells left untreated or treated with 40 μm of cycloheximide for 2, 4, 6 and 8 hours. Cell lysate (50 μg) were electrophoresed on 10 % SDS-PAGE. Blots were probed with anti-Osterix-antibodies (top) and anti-GAPDH antibodies (bottom) to verify equal protein loading. C. Bar graphs represent Osterix/GAPDH of three pooled experiments; * p<0.01 Vs. WT. D. Representative 20× fluorescent images of Osterix-expressing WT and YF periosteal cells counterstained with DAPI in the presence and absence of 10μm LY294002. White arrowheads point to cells in which Osterix is localized in the nucleus. E. Bar graphs represent the percent of periosteal cells with nuclear Osterix expression over total number of cells in the absence and presence of 1 and 10 μM LY294002. p* p<0.01 Vs. WT. A representative of 3 experiments is shown.
Imagelab, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A. Periosteal cell cultures were left untreated or were treated with 1 or 10 μm of LY294002 for 60 minutes. Total cell lysate (100 μg) was electrophoresed on 8 % SDS-PAGE. The blot was probed using anti-phospho AKT Thr 308 antibody (top), and then stripped and reprobed with anti-AKT (bottom) to verify equal loading of protein. The amounts of phosphorylated to total proteins in individual bands were quantified by using Odyssey Infrared Imaging Systems software 2.1 (LICOR Biosciences), and the ratio of pAKT/AKT is indicated. A representative of two experiments is shown. B. Cells left untreated or treated with 40 μm of cycloheximide for 2, 4, 6 and 8 hours. Cell lysate (50 μg) were electrophoresed on 10 % SDS-PAGE. Blots were probed with anti-Osterix-antibodies (top) and anti-GAPDH antibodies (bottom) to verify equal protein loading. C. Bar graphs represent Osterix/GAPDH of three pooled experiments; * p<0.01 Vs. WT. D. Representative 20× fluorescent images of Osterix-expressing WT and YF periosteal cells counterstained with DAPI in the presence and absence of 10μm LY294002. White arrowheads point to cells in which Osterix is localized in the nucleus. E. Bar graphs represent the percent of periosteal cells with nuclear Osterix expression over total number of cells in the absence and presence of 1 and 10 μM LY294002. p* p<0.01 Vs. WT. A representative of 3 experiments is shown.

Journal: Bone

Article Title: Loss of Cbl-PI3K Interaction Modulates the Periosteal Response to Fracture by Enhancing Osteogenic Commitment and Differentiation

doi: 10.1016/j.bone.2016.11.020

Figure Lengend Snippet: A. Periosteal cell cultures were left untreated or were treated with 1 or 10 μm of LY294002 for 60 minutes. Total cell lysate (100 μg) was electrophoresed on 8 % SDS-PAGE. The blot was probed using anti-phospho AKT Thr 308 antibody (top), and then stripped and reprobed with anti-AKT (bottom) to verify equal loading of protein. The amounts of phosphorylated to total proteins in individual bands were quantified by using Odyssey Infrared Imaging Systems software 2.1 (LICOR Biosciences), and the ratio of pAKT/AKT is indicated. A representative of two experiments is shown. B. Cells left untreated or treated with 40 μm of cycloheximide for 2, 4, 6 and 8 hours. Cell lysate (50 μg) were electrophoresed on 10 % SDS-PAGE. Blots were probed with anti-Osterix-antibodies (top) and anti-GAPDH antibodies (bottom) to verify equal protein loading. C. Bar graphs represent Osterix/GAPDH of three pooled experiments; * p<0.01 Vs. WT. D. Representative 20× fluorescent images of Osterix-expressing WT and YF periosteal cells counterstained with DAPI in the presence and absence of 10μm LY294002. White arrowheads point to cells in which Osterix is localized in the nucleus. E. Bar graphs represent the percent of periosteal cells with nuclear Osterix expression over total number of cells in the absence and presence of 1 and 10 μM LY294002. p* p<0.01 Vs. WT. A representative of 3 experiments is shown.

Article Snippet: The amounts of proteins in individual bands were quantified by using Odyssey Infrared Imaging Systems software 2.1 (LICOR Biosciences, Lincoln, NE) as previously reported [ 23 , 25 ].

Techniques: SDS Page, Imaging, Software, Expressing